OcupathIF
AI-Powered Fluorescence
Microscopy Analysis

From Raw Image to Insight — In Minutes

OcupathIF is a desktop application that automates cell segmentation, phenotyping, and spatial analysis for multi-channel fluorescence microscopy images — fast to run, fully reviewable at every step, and configurable to your panel and your research questions.

macOS · WindowsMulti-channel IF imagesOne-click installNo code required
OcupathIF fluorescence microscopy
OcupathIF
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[ core advantages ]

Analysis that's fast, flexible,
customizable, and fully reviewable

OcupathIF runs the full pipeline automatically, adapts to your panel and your questions, and keeps every result open for inspection.

10x Faster Analysis

From raw image to result in minutes, not hours — parallel tile processing and progressive loading keep large whole-slide images tractable.

  • < 5s image preview load
  • Pipeline speedup — 1GB mIF image (~5mm² tissue area, 8 channels mIF) analyzed in 5 minutes*
  • 5-step straightforward, guided workflow — View → Tissue → Cells → Phenotype & Spatial → Export

*Based on internal CPU testing.

Fast

Flexible & Customizable

Configure each analysis for your panel, tissue, and study goals.

  • Adjust analysis — Regions, thresholds, phenotypes, and spatial queries
  • Reuse settings — Apply saved configurations across images and batches
  • Custom support — Request tailored workflows, analyses, or export formats
Customizable

Straightforward & Interactive

A visual, click-first workflow lets researchers move from image to results with minimal training and no coding.

  • Intuitive UI/UX — Clear controls and practical defaults
  • Interactive viewer — Pan, zoom, and switch markers in one click
  • Edit on the image — Adjust regions, cell boundaries, and gates directly
  • Guided workflow — Move from View to Export with immediate visual feedback
No code required

One workflow, five stages

OcupathIF moves with you from the raw image to exportable evidence — each stage reviewable before the next begins.

Seamless Image Viewing

  • Automatic channel detection : reads excitation/emission wavelengths and fluorophore names directly from OME metadata (qptiff, OME-TIFF), no manual relabeling
  • Thumbnail-first progressive loading (~3s preview), even on large whole-slide images
  • Broad format support : opens .qptiff (Akoya Vectra/Polaris), .ome.tiff/.ome.tif, .tiff/.tif, .svs, .scn, .ndpi, .mrxs, and DICOM WSI (.dcm/.dicom) directly
Getting Started

The fastest path from IF image to insight.
Free, no code required.

macOS & Windows · Free · No code required